EV Winter School 2019

WINTER SCHOOL

Basics to Work with

EXTRACELLULAR VESICLES

December 2-5/ 2019

RAVILA 14B, TARTU, eSTONIA

PROGRAM

9.00-9.20Welcoming and Introduction | Alireza Fazeli   
at Ravila 14b, 2nd floor seminar room
9.20-10.45Lecture 1 A guide to working with extracellular vesicles: minimal experimental and reporting requirements | Janeli Viil 
at Ravila 14b, 2nd floor seminar room
10.45-11.00Tea & Coffee break 
at Ravila 14b, 2nd floor seminar room
11.00-12.00Lecture 2 Methods for EV purification | Getnet Balcha Midekessa, Danilo Mladenoviċ 
at Ravila 14b, 2nd floor seminar room
12.00-12.40Lunch 
at Ravila 14b, 3rd floor room
12.40-14.40Workshop 1 Introduction to Size Exclusion-High Performance Liquid Chromatography (SEC-HPLC) and Tangential Flow Filtration (TFF) | Getnet Balcha Midekessa, Danilo Mladenoviċ, Keerthie Dissanayake 
at Ravila 14b, 1st floor lab
14.40-14.50Tea & Coffee break
at Ravila 14b, 2nd floor seminar room
14.50-17.00Workshop 2 EV purification from follicular fluid samples | Mohammad Mehedi Hasan, Qurat Ul Ain Reshi
at Ravila 14b, 1st floor lab
9.00-10.00Lecture 3 Introduction to Zetaview NTA | Sascha Raschke
at Ravila 14b, 2nd floor seminar room
10.00-13.00

Workshop 3 EV size and concentration measurements with NTA | Keerthie Dissanayake      at Ravila 14b, 1st floor lab

11.00- .. Tea & Coffee break (at Ravila 14b, 2nd floor seminar room)

Workshop 4 EV zeta-potential measurements with NTA | Getnet Balcha Midekessa                at Ravila 14b, 1st floor lab

13.00-14.00Lunch
at Ravila 14b, 3rd floor room
14.00-15.00Lecture 4 (open seminar) Potentials and Capabilities of the Extracellular Vesicle (EV) Array – in health and disease | Malene Møller Jørgensen
at Ravila 14a, Chemicum, 1st floor seminar room 1100
15.00-15.10Tea & Coffee break
at Ravila 14b, 2nd floor seminar room
15.10-17.10Workshop 5 NTA data analysis | Freddy Lättekivi
at
 Ravila 14b, 2nd floor seminar room
19.00-..Dinner at restaurant Kolmtilli
9.00-10.15Lecture 5 Transmission Electron Microscopy (TEM) | Aneta  Andronowska
at Ravila 14b, 2nd floor seminar room
10.15-11.30Lecture 6 High-resolution microscopy and its potential applications in EV research | Sergei Kopantsuk
at Ravila 14b, 2nd floor seminar room
11.30-11.40Tea & Coffee break
at Ravila 14b, 2nd floor seminar room
11.40-12.10Lecture 7 RNA extraction from EVs | Kasun Godakumara
at Ravila 14b, 2nd floor seminar room
12.10-13.00Workshop 6 (part 1) RNA extraction from EVs | Kasun Godakumara
at Ravila 14b, 1st floor lab
13.00-13.40Lunch
at Ravila 14b, 3rd floor room
13.40-17.00Workshop 6 (part 2) RNA extraction from EVs | Kasun Godakumara
at Ravila 14b, 1st floor lab
10.00 – 13.00Gamerz tour in Tartu 
starting from Tartu Town Hall Square
13.00 – 17.00

Cooking & eating together 
at Sisevete Saatkond on River Emajõgi

 

VENUE

ABOUT

Winter school aims to introduce the basic methods for working with Extracellular Vesicles (EVs) The EV Winter School will go through all the steps to work with EVs starting from sample preparation and EV purification until the data analysis of different EV quality and quantity data. A series of seminars and hands-on training will be provided, thus a participant would have a thorough overview of different methods applicable at the University of Tartu (UT). 

The seminars and hands-on training will be held by researchers and Ph.D. students working on different EV projects under the ERA Chair of Translational Genomics at the University of Tartu. Additionally, at least two experts from outside the UT will be involved. The outcome of this Winter School would be knowledge, which helps a student or researcher to plan and conduct a project to study EVs. 

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The course will be conducted by the ERA Chair of Translational Genomics at the University of Tartu (European Union’s Horizon 2020 research and innovation program under grant agreement No 668989).