WINTER SCHOOL
Basics to Work with
EXTRACELLULAR VESICLES
December 2-5/ 2019
RAVILA 14B, TARTU, eSTONIA
PROGRAM
| 9.00-9.20 | Welcoming and Introduction | Alireza Fazeli at Ravila 14b, 2nd floor seminar room |
| 9.20-10.45 | Lecture 1 A guide to working with extracellular vesicles: minimal experimental and reporting requirements | Janeli Viil at Ravila 14b, 2nd floor seminar room |
| 10.45-11.00 | Tea & Coffee break at Ravila 14b, 2nd floor seminar room |
| 11.00-12.00 | Lecture 2 Methods for EV purification | Getnet Balcha Midekessa, Danilo Mladenoviċ at Ravila 14b, 2nd floor seminar room |
| 12.00-12.40 | Lunch at Ravila 14b, 3rd floor room |
| 12.40-14.40 | Workshop 1 Introduction to Size Exclusion-High Performance Liquid Chromatography (SEC-HPLC) and Tangential Flow Filtration (TFF) | Getnet Balcha Midekessa, Danilo Mladenoviċ, Keerthie Dissanayake at Ravila 14b, 1st floor lab |
| 14.40-14.50 | Tea & Coffee break at Ravila 14b, 2nd floor seminar room |
| 14.50-17.00 | Workshop 2 EV purification from follicular fluid samples | Mohammad Mehedi Hasan, Qurat Ul Ain Reshi at Ravila 14b, 1st floor lab |
| 9.00-10.00 | Lecture 3 Introduction to Zetaview NTA | Sascha Raschke at Ravila 14b, 2nd floor seminar room |
| 10.00-13.00 | Workshop 3 EV size and concentration measurements with NTA | Keerthie Dissanayake at Ravila 14b, 1st floor lab 11.00- .. Tea & Coffee break (at Ravila 14b, 2nd floor seminar room) Workshop 4 EV zeta-potential measurements with NTA | Getnet Balcha Midekessa at Ravila 14b, 1st floor lab |
| 13.00-14.00 | Lunch at Ravila 14b, 3rd floor room |
| 14.00-15.00 | Lecture 4 (open seminar) Potentials and Capabilities of the Extracellular Vesicle (EV) Array – in health and disease | Malene Møller Jørgensen at Ravila 14a, Chemicum, 1st floor seminar room 1100 |
| 15.00-15.10 | Tea & Coffee break at Ravila 14b, 2nd floor seminar room |
| 15.10-17.10 | Workshop 5 NTA data analysis | Freddy Lättekivi at Ravila 14b, 2nd floor seminar room |
| 19.00-.. | Dinner at restaurant Kolmtilli |
| 9.00-10.15 | Lecture 5 Transmission Electron Microscopy (TEM) | Aneta Andronowska at Ravila 14b, 2nd floor seminar room |
| 10.15-11.30 | Lecture 6 High-resolution microscopy and its potential applications in EV research | Sergei Kopantsuk at Ravila 14b, 2nd floor seminar room |
| 11.30-11.40 | Tea & Coffee break at Ravila 14b, 2nd floor seminar room |
| 11.40-12.10 | Lecture 7 RNA extraction from EVs | Kasun Godakumara at Ravila 14b, 2nd floor seminar room |
| 12.10-13.00 | Workshop 6 (part 1) RNA extraction from EVs | Kasun Godakumara at Ravila 14b, 1st floor lab |
| 13.00-13.40 | Lunch at Ravila 14b, 3rd floor room |
| 13.40-17.00 | Workshop 6 (part 2) RNA extraction from EVs | Kasun Godakumara at Ravila 14b, 1st floor lab |
| 10.00 – 13.00 | Gamerz tour in Tartu starting from Tartu Town Hall Square |
| 13.00 – 17.00 | Cooking & eating together |
VENUE
ABOUT
Winter school aims to introduce the basic methods for working with Extracellular Vesicles (EVs) The EV Winter School will go through all the steps to work with EVs starting from sample preparation and EV purification until the data analysis of different EV quality and quantity data. A series of seminars and hands-on training will be provided, thus a participant would have a thorough overview of different methods applicable at the University of Tartu (UT).
The seminars and hands-on training will be held by researchers and Ph.D. students working on different EV projects under the ERA Chair of Translational Genomics at the University of Tartu. Additionally, at least two experts from outside the UT will be involved. The outcome of this Winter School would be knowledge, which helps a student or researcher to plan and conduct a project to study EVs.
The course will be conducted by the ERA Chair of Translational Genomics at the University of Tartu (European Union’s Horizon 2020 research and innovation program under grant agreement No 668989).